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Albert Cardona

Quite the big deal:

"Light-field deep learning enables high-throughput, scattering-mitigated calcium imaging", by Howe et al. 2025 (Amanda Foust lab).
biorxiv.org/content/10.1101/20

Transfers one-photon light field images of Ca2+ sensors monitoring neuronal activity, which suffer from scattering in the mouse brain, to two-photon volumes that don't, using machine learning.

Image volumes acquired at 100 Hz demonstrate 10Hz spike rates.

The dual-mode LFM combines a 2P laser-scanning path with a one-photon LFM. A removable dichroic mirror diverts fluorescence to the photomuliplier tube (PMT) for 2P collection. When the 2P dichroic is disengaged, fluorescence is imaged by the LFM with a microlens array (MLA). Distances not to scale.
#neuroscience#mouse#CalciumImaging
Mar 26, 2025, 04:01 PM·Public
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